Growth Tips

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Liquid media culturing

  • Mesoplasma florum L1 grows to high visible density in ATCC 1161 overnight
    • Does not settle at bottom as quickly as E coli, B subtilis, or S cerevisiae

Glycerol stocking

  • From an overnight Mesoplasma florum culture, add 500ul of culture to 500ul of 50% glycerol

Growth tips

Mesoplasma florum under stereo microscope.
  • Mesoplasma florum L1 creates pickable colonies within 48 hours
    • Small colonies are visible within 24 hours, however liquid cultures are cloudy after 1 day of growth
    • When spreading on plates for liquid culture, remember that liquid cultures of Mesoplasma florum tend to have more CFUs per ml than most other bacteria, so spread sparingly (KG 2019-08-23)
    • Colonies are small, barely visible to naked eye, with fried egg morphology
      • Recommended to use a stereo microscope to find good colonies
  • Mesoplasma florum W37 creates pickable colonies within 72 hours
    • More info later
  • Can avoid making separate ATCC 1161 media by combining 4:1 1x liquid media with 5% agarose. Plates are still a bit soft but easier to make (KG 2016-10-14)
  • Bugs are very attracted to horse serum. If working with in a DIY lab space, make sure to seal all petri dishes and flasks

    • DMEM media
    • SOB + Tween80
      • Venter group has had success using SOB with serum and glucose [1], perhaps I just require glucose and serum or perhaps glucose and TWEEN80? Would make for far simpler broth.


Mesoplasma florum L1 on a plate (right) with 15ug tetracycline. Positive control on left.
  • Does not grow in 15ug tetracycline [2]


  1. Carole Lartigue et al (2007). "Genome Transplantation in Bacteria: Changing One Species to Another". Science. Quote from article- "We prepared the M. capricolum recipient cells in a 6-ml culture of SOB medium (22) containing 17% fetal bovine serum and 0.5% glucose". [1]
  2. Dominick Matteau et al (2017). "Development of oriC-Based Plasmids for Mesoplasma florum". Applied and Environmental Microbiology. Quote from article- "Unless specified, tetracycline was used at 15 μg/ml for either E. coli or M. florum". [2]