Media

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ATCC 1161 Medium

Heart Infusion Broth 8.75g
Sucrose 20g
Yeast extract 6.75g
Heat inactivated Horse serum 100ml
Agar (OPTIONAL) 6g
Phenol Red (0.5%) 4ml
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Total volume: 500ml


  1. Add 8.75 Heart Infusion Broth powder, 20g sucrose, 6.75g yeast extract, to 500ml H20. Optionally add 6g agar at this step.
  2. Autoclave mixture. Heat Horse serum to approximately 50c
  3. Cool autoclaved media to ~55c
  4. Add 100ml Horse serum sterilely
  5. Add 4ml phenol red to final media


KG 1161 Medium

Heart Infusion Broth 8.75g
Sucrose 20g
Yeast extract 6.75g
Tween80 200ul
Palmitic acid 0.005g [1]
Agar (OPTIONAL) 6g
Phenol Red (0.5%) 4ml
------------------
Total volume: 500ml
  1. Add 8.75 Heart Infusion Broth powder, 20g sucrose, 6.75g yeast extract, to 500ml H20. Optionally add 6g agar at this step.
  2. Autoclave mixture
  3. Cool autoclaved media to ~55c (if adding antibiotics)
  4. Add 4ml phenol red to final media
  5. Add 200ul of Tween80 to final media

References

  1. McCoy et al (1984). "Acholeplasma florum, a New Species Isolated from Plants". Microbiology society. [[1]]